Dynamic Metabolic Changes in Arabidopsis Seedlings under Hypoxia Stress and Subsequent Reoxygenation Recovery

Hypoxic stress, caused by the low cellular oxygen in events of flooding or waterlogging, limits crop productivity many regions world. stress plants is often dynamic and followed a reoxygenation process that returns level to normal. Although metabolic responses hypoxia have been studied plants, less known about recovery processes following removal. To better understand shift from low-oxygen environment reoxygenated environment, we performed time-course measurements metabolites Arabidopsis seedlings at 0, 6, 12, 24 h after (100% N2 environment). Among 80 features characterized using GC-MS, 60% them were significantly changed under hypoxia. The phase was accompanied progressively fewer changes. Only 26% reoxygenation. Hypoxia-induced changes returned normal levels different speeds. For example, hypoxia-induced accumulation lactate decreased basal 6 reoxygenation, whereas alanine GABA showed partial Some metabolites, such as gluconate, xylose, guanine, adenosine, constantly increased during These demonstrate flexibility complexity plant metabolism subsequent recovery.